Evaluation of In Vitro Wound Healing Activity in the Ethanolic Extract of Aristolochia Bracteolata

Abstract

In our bodies, healing from wounds is a biological process that takes time. Medicinal plants heal wounds more quickly than the body's natural processes. One of the medicinal plants, Aristolochia bracteloata, is used to cure skin conditions, inflammation, and more. The plant's leaves are harvested and dried for two weeks in the shade. The leaves were thoroughly pulverised after being shade-dried, and extract was made using the soxhelt method with 70% ethanol as the solvent. Analyses of the extract's qualitative phytochemical composition were conducted. The plant extract contains saponins, glycosides, carbohydrates, phenols, courmarins, tannins, flavonoids, and anthocyanins, among other phytochemicals. Nevertheless, the leaf extract was discovered to be devoid of proteins, alkaloids, phytosterols, steroids, and terpenoids. The agar with the well diffusion method was used to test the extract's antibacterial and antifungal properties, and the extract displayed the zone of inhibition. The DPPH assay was used to test the antioxidant activity, and the extract showed free radical scavenging capabilities. The considerable acceleration of wound healing in the human cell line is investigated using the ethanolic extract of Aristolochia bracteolate. In L929 cells, the plant extract heals the lesion after 24 hours. In the wound healing scratch assay, the low concentration of the extract heals the wound more quickly than the high concentration of the extract.