Antioxidant and Antimicrobial Activity of Silver Nanoparticles Biosynthesized Using Leptodenia Pyrotechnicaaerial Part

Abstract

Nanotechnology is considered as a fast and constantly growing field in the biomedical and healthcare applications due to its relatively small size and vast advantages. Researches have proven that the silver nanoparticle synthesized from the green route has an excellent antimicrobial and antioxidant properties and is free of any adverse effects.In the present study the antimicrobial and antioxidant activity of Leptodeniapyrotechnica aerial part and silver nanoparticle (AgNPs) synthesized by Leptodeniapyrotechnica aerial part (LPA-AgNPs) have been investigated comparatively. The characterization of AgNPs was done by using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy. The antimicrobial activity of LPA-AgNPs and methanol extract of L. pyrotechnica aerial part (LPAM) were tested against bacterial strains (Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli) and fungal strains (Candida albicans, Aspergillus niger, Trichoderma reeseiand Penicilliumchrysogenum) using the agar well diffusion method. And the catalase (CAT), peroxidase (POD) and ferric reducing antioxidant power (FRAP) assay also evaluated. The prominent transmission band in FTIR spectra was observed at 3443 cm^-1 (hydroxy group, H-bonded OH stretch) and at 1638 cm^-1 (alkenyl C=C stretch). In SEM characterization, the nanoparticles were in spherical shape with 23-86 nmrange of particle size. LPA-AgNPs showed highest antibacterial activity against B. subtilis, P. aeruginosa, E. coli and S. aureus with 33 mm, 17 mm, 14 mm and 20 mm inhibition zone, respectively in comparison with LPAM. Likewise antifungal properties of LPA-AgNPs were significant against Trichoderma reesei and Aspergillus niger with 12 mm inhibition diameter comparatively. The highest CAT (0.089µM H₂O₂ reduce/gm Fwt/sec), POD (0.657µM/L/gm dwt/sec) and FRAP (1103.21 µM) activity was also observed with green synthesized AgNPs. It can be concluded from the results that the silver nanoparticle synthesized from the Leptodeniapyrotechnica aerial part washighest antimicrobial and antioxidant activities as compared to Leptodeniapyrotechnica aerial part.